Fig. 6.
MALDI-TOF imaging of the marine sponge Dysidea herbaceae. (A) MALDI target plate with thin section in place. (B) Autofluorescence of D. herbaceae thin section at 590 nm+. (C) Autofluorescence of D. herbaceae thin section at 420 nm+. (D) MALDI image of m/z 530. This mass and the complex molecular ion isotope cluster suggests its identity as the hexachlorinated peptide 13-demethylisodysidenin (20). (E) Autofluorescence (420 nm) image overlaid by MALDI image at m/z 530 indicating localization of compound with this mass. (F) MALDI image of unknown compound m/z 1,028. (G) Autofluorescence (420 nm) image overlaid with m/z 530 (red) and m/z 1,028 (green) showing the differential localization of these two molecules.