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. 2008 Mar 24;180(6):1073–1086. doi: 10.1083/jcb.200706009

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Copyright © 2008, The Rockefeller University Press

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Figure 6. — Replication fork pause plays a role in suppression of HU sensitivity of chk1Δ dun1Δ cells. (A) Cells containing the indicated episome were spotted onto YPD medium containing the indicated concentration of HU (in millimoles). (B) Suppression of checkpoint mutant HU sensitivity by an episomal replication origin is independent of spindle dynamics regulation.Cells containing either p2μ (pRS426) or pARS (pRS416) were spotted onto YPD medium containing the indicated concentration of HU (in millimoles). (C) Cells were released from G1 into medium containing 50 mM HU, fixed, and stained with DAPI and anti-tubulin to visualize nuclei and spindles. For each strain, 100 cells for each time point were scored as having either short (<3 μm) or elongated (≥3 μm) spindles. Data points indicate the percentage of cells with elongated spindles. (D–F) The episomal replication fork pause is critical to restore viability to chk1Δ dun1Δ cells.Cells containing the indicated episomes were spotted onto YPD medium containing the indicated concentration of HU (in millimoles). (A and D, bottom) Schematics of episomes used in these studies.