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. 2008 Mar 24;180(6):1115–1131. doi: 10.1083/jcb.200708170

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Copyright © 2008, The Rockefeller University Press

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Figure 1. — Mitotic nuclear accumulation of condensin and its loss in two mutants. (A) Time-lapse images of the wild-type S. pombe cultured at 36°C. Cells expressed the condensin subunit Cnd1-GFP (green) and the SPB and nuclear envelope protein Sad1-RFP (red). In the inset, the arrowheads indicate the two enriched nuclear domains. The arrow in the merged images shows the condensin in the middle of the two separated daughter nuclei. (bottom) The mitotic nucleus containing Cut14-GFP and stained with the ethidium bromide–DAPI mixture, which revealed the nucleolar domain in the mitotic nucleus. (B and C) Two example cells in metaphase at 1-min intervals are shown that expressed Cnd1-GFP Sad1-RFP (B) or Cut14-GFP Sad1-RFP (C). (D) Time-lapse images for a wild-type cell carrying plasmid REP81-Cut3WT (left) or REP81-Cut3 T19A (right). (E and F) Time-lapse images of mutant cut15-85 that expressed Cut14-GFP (E) or Cnd1-GFP (F) and Sad1-mCherry. (A and D–F) The numbers in the panels indicate time in minutes. Bars: (A, top; and B–F) 10 μm; (A, bottom) 2 μm.