Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Mar 24;180(6):1115–1131. doi: 10.1083/jcb.200708170

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Enrichment of condensin at rDNAs requires a nucleolar protein, Acr1, which interacts with the rDNA upstream activator complex. (A) Cut14-GFP was observed in mitotic cells of the acr1-936 mutant and the control wild type cultured at 36°C for 4 h. (B) A ChIP assay was performed using extracts of asynchronous wild-type and acr1-936 mutant that expressed Cut14-Flag. The wild-type and acr1-936 mutant were cultured at 26°C and shifted to 36°C for 4 h, and immunoprecipitation was performed. Real-time PCR was used to amplify and quantify DNAs with the PCR primers (N1, N2, N3, lys1 ⁺, and cnt1). Error bars represent SD. (C) Localization of Acr1 was examined in the strain that expressed Acr1-GFP. (D) A ChIP assay was performed using the strain that expressed Acr1-Flag. Two rDNA probes (N2 and 18S) and four control probes (cnt1, imr1, dg, and lys1 ⁺) were used. (E, top) Preparation of proteins coprecipitated with Acr1-Flag and the Flag-only control for liquid chromatography–tandem mass spectrometry. The positions of identified protein names are indicated. The two bands were obtained for Acr1-Flag (Fig. S2 D, available at http://www.jcb.org/cgi/content/full/jcb.200708170/DC1). (bottom) Four proteins (Rrn5, Rrn11, Rrn7, and Spp27) were coprecipitated with Acr1-Flag. emPAI, exponentially modified protein abundance index (Ishihama et al., 2005). Bars: (A) 2 μm; (C) 10 μm.