Figure 7.

Condensin entered the nucleus but failed to accumulate at the two distinct domains in the cut17 mutant and the dominant-negative Ark1/aurora cells. (A, top) Wild-type and cut17-275 mutant cells that expressed Cut14-GFP and Sad1-mCherry were cultured at 26°C, shifted to 36°C for 3 h, and observed. (bottom) The wild-type cells carrying the inducible plasmid REP1-Ark1WT or REP1-Ark1-K118R were cultured in the absence of thiamine at 26°C. (B) Time-lapse series of the Cut14-GFP and Sad1-mCherry images in the wild-type or cut17-275 cells cultured at 36°C for 3 h (top) and in the cells expressing wild-type Ark1 or the dominant-negative Ark1 K118R are shown. Bars, 10 μm.