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. 2008 Mar 24;180(6):1149–1161. doi: 10.1083/jcb.200707196

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Copyright © 2008, The Rockefeller University Press

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Figure 2. — Cotranslational translocation defects of yeast that express SRβ-ΔTM. (A–D) Wild-type (wt) and mutant yeast cells were grown to mid-log phase at 30°C in SEG media. The cultures were diluted into SD media and allowed to grow for 4 h (A, C, and D) or 24 h (B) at 30°C. Wild-type or mutant cells (4 A₆₀₀) were collected and pulse labeled for 7 min. DPAPB immunoprecipitates was resolved by SDS-PAGE. Glycosylated (DPAPB) and nonglycosylated (pDPAPB) forms of DPAPB were quantified with a molecular imager to calculate the percent precursor. Plasmids encoding the soluble SR (SRα + SRβ-ΔTM) and the Ssh1 complex are designated as SR-ΔTM and SSH1*, respectively. (A and B) Total incorporation of Tran-³⁵S-label (pDPAPB + DPAPB) in each strain is expressed relative to the wild-type strain.