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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Peptides. 2007 Dec 5;29(2):179–185. doi: 10.1016/j.peptides.2007.09.021

Figure 1.

Figure 1

Purification of peptides as14a and as14b. Panel A) Fractionation of the venom extract (~1 mg total protein) by an analytical C18 column eluted with an isocratic step at 5% solution B for 10 min followed by a linear gradient from 5–55% solution B over 100 min at 1 ml/min. HPLC solutions were: 0.1% (v/v) TFA in water (solution A), and 0.085% (v/v) TFA in 90% aqueous ACN (solution B). The absorbance was monitored at 220 nm. The peaks highlighted by arrows in panel A were further purified on the same column, using an isocratic step (20% solution B for 10 min) and then increasing solution B by 0.25%/min at 1 ml/min (as14a, panel B; as14b, panel D). The peaks highlighted by arrows in panels B (as14a) and D (as14b) were further purified on an analytical C8 column using the same elution procedure as above (as14a, panel C; as14b, panel E).