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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Peptides. 2007 Dec 5;29(2):186–195. doi: 10.1016/j.peptides.2007.09.022

Figure 1.

Figure 1

Purification of conorfamide-Sr2. (A) Fractionation of the crude extract from the venom ducts of Conus spurius by a semipreparative Vydac C18 column. The elution was conducted at room temperature with a linear gradient from 5 to 100% solution B over 95 min (dashed line) at 2.5 ml/min. HPLC solutions were: 0.1% (v/v) TFA in water (solution A), and 0.085% (v/v) TFA in 90% (v/v) aqueous MeCN (solution B). The absorbance was monitored at 220 nm. (B) The peak highlighted by the arrow in (A) was further purified on a semipreparative Vydac C8 column using a linear gradient from 24% to 45% solution B over 63 min after an isocratic step at 24% solution B for 15 min (dashed line), at 1 ml/min. (C) The peak highlighted by the arrow in (B) was rechromatographed on an analytical Agilent C8 column using a linear gradient from 45% to 55% solution B (0.1% (v/v) TFA in 60% (v/v) aqueous MeCN) over 40 min (dashed line), at 1 ml/min. The gradients are displayed at the same scale (right side).