Inhibition of caspase 8 induced apoptosis by LAT constructs. Using our standard apoptosis blocking assay [15, 16] Neuro2A cells were co-transfected with a caspase 8 expressing plasmid to induce apoptosis and one of the LAT expressing plasmids (LAT3.3A, LAT3.3E, LAT3.3I, or LAT3.3U). Control cells were transfected with the same total amount of empty plasmid. Caspase 8 cells were co-transfected with the caspase 8 plasmid and the same amount of empty plasmid used in the various LAT samples. Each bar represents the average +/− SE of 3 independent experiments, each done at least in triplicate.