Figure 3.

Heterologous expression of xylanase gene. Activity plate assays with Azur-linked Xylan (AZCL, Megazyme). The two top panels show assays using extracts of yeast cells transformed with an empty vector (pYES2), which was expected to give no reaction. The two bottom panels show assays using extracts of yeast cells transformed with cDNA of LgXyn1 inserted in pYES2. These show a clear indication of xylanase activity, with the strongest activity being observed when the yeast cells were grown on galactose-containing media, which induces transcription of the inserted cDNA.