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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Anal Biochem. 2007 Dec 25;375(1):18–26. doi: 10.1016/j.ab.2007.12.023

Fig. 1.

Fig. 1

Basic strategy for immobilization, kinase reaction and immunodetection. Peptides are tethered to a hydrogel in multiwell plates via multivalent crosslinkers, subjected to phosphorylation by kinases, and then interrogated with anti-phosphotyrosine antibody and secondary antibody-horseradish peroxidase conjugate with detection by chemifluorescence.