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. 2008 Mar 28;105(14):5603–5608. doi: 10.1073/pnas.0801395105

Fig. 1.

Fig. 1.

Presynaptic clusters during postnatal development. (A) Confocal z-stack projections show the distribution of VGLUT label in the medial half of the MNTB at P0, P3, and P15. Lateral is right, dorsal is up. (B) Perisomatic labeling of VGLUT-positive clusters is revealed in triple-stained tissue. (Top) Z-stack projection of calyces that were labeled with a fluorescent dextran (gray scale). (Middle and Bottom) Single confocal sections from the same focal plane demonstrating the location of the postsynaptic cell (counterstained with SYTOX, color-coded blue) and the presynaptic clusters (color-coded red), respectively. (C) The fraction of perisomatic LPCs in medial and lateral regions of the MNTB increased during postnatal development. (D) Percentage of large axosomatic contacts that were colabeled with an LPC at P3 (31 contacts from three animals) and in control sections at P8 (29 contacts from one animal). (E) Density of presynaptic clusters in labeled axons at P1 and P3. (F) Cumulative histogram of presynaptic cluster volume for axons at P1 and P3 (blue and red, respectively) and for calyces of Held at P3 (cyan; 31 labeled axons). Confocal images were contrasted with Adobe Photoshop.