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. 2008 Mar 28;105(14):5603–5608. doi: 10.1073/pnas.0801395105

Fig. 3.

Fig. 3.

In vivo two-photon imaging in the MNTB of neonate rats. (A) Exemplar image of labeled axons at P3. (Inset) Location of a calyx of Held terminal. (B) Close-up view of the labeled calyx in A. (C) Axon collateral length was traced in at least three z-stacks from different animals and plotted as a cumulative histogram. (D) Cumulative histogram of the number of collaterals per 100 μm of axon. Two-photon images are collapsed z-stacks of >50 optical slices acquired at 1-μm intervals.