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Calyx of Held collaterals are pruned during postnatal development. Axons were labeled in brain slices, fixed, and imaged with a confocal microscope. The length and number of collaterals were traced at different ages. (A) Cumulative histogram of collateral length shows a gradual decrease in collateral length between P3 and P16. (B) Cumulative histogram of the number of branches per calyx shows abrupt changes between P13 and P16.
