Fig. 4.

EBNA-6 and S18-2 are components of the one protein complex in vivo. For immunoprecipitations, 5 μg of antibodies was coupled to the CN-Br Sepharose, and such supports were used to capture the corresponding proteins from the Nonidet P-40 LCL cell lysates. After extensive washing, the protein complexes were eluted by heat and separated on the polyacrylamide gel. Beads not coupled to antibodies were used as a negative control. Ten micrograms of LCL lysate was used as a control for protein expression. (Left) EBNA-6 from LCL was precipitated by anti-S18-2 antibody (with two clones, 75-5 and 76-2). The EBNA-6 antibody was used to probe the membrane. (Right) Precipitation of S18-2 from LCL by the anti-EBNA-6 mouse antibody. Clone 76-2 was used as a probe for Western blot analysis. Notice that beads not coupled to antibodies did not give any signal.