Fig. 3.

Cross-linking analysis of wild-type and mutant hGITRL. After cross-linking using BS3, equal amounts of samples were loaded for SDS/PAGE analysis. Sample names were labeled on the upper side. Note that wild-type hGITRL can form high-molecular-weight superclusters binding to hGITR, whereas mutant hGITRL forms can only form dimer and shows weak receptor binding. Different oligomeric states of hGITRL were labeled. WT-hGITRL, wild-type hGITRL; Cdel-hGITRL, C-terminal deletion mutant hGITRL; WT-hGITR, wild-type hGITR.