Fig. 6.

Deglycosylation of CD11a/CD18-expressing Jurkat cells decreases cytotoxic activity of LtxA of A. actinomycetemcomitans and of HlyA of E. coli. Jurkat cells, expressing the CD11a/CD18 integrin complex on the cell surface, were treated or not with a mixture of PNGase F, Endo H, and neuraminidase at 37°C overnight. Next, protein-content-equalized mock or LtxA-containing bacterial lysates (A) and mock or HlyA-containing culture supernatants (B) were added to the cells for the indicated times, and cell viability was determined by using trypan blue dye exclusion assay (see Materials and Methods). The data are representative of three independent experiments ± SD.