Fig. 1.
Stability of the Polδ holoenzyme assembled with PCNA or K164–Ub–PCNA. (A) The processive DNA synthesis by Polδ holoenzyme assembled with PCNA or K164–Ub–PCNA in the presence of increasing PolδAA trap concentration is shown. The experiment was carried out under stalled condition (see Materials and Methods). (B) The DNA synthesis by Polδ holoenzyme assembled with PCNA (black bars) or K164–Ub–PCNA (gray bars) after 1, 5, and 10 min of incubation with ×10 PolδAA trap is shown.