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The thiolated uridine-34 is absent in a strain deleted for ctu1. Mass chromatograms for mcm5S2U (Upper) and mcm5 (Lower) of purified tRNAs from wild type (Left) and a strain deleted for ctu1 (Right) with relative abundance (y axis) and time in minutes (x axis). In each Inset, the mass spectrum of the protonated molecule and the protonated free base corresponding to the observed peak is presented with the chemical structure of the nucleoside detected.
