Fig. 4.
De novo-synthesized LPS requires LptFG to reach the cell surface. LptFG-diploid strain NR1111 grown in the presence of arabinose (lane 1) was treated with EDTA (lane 2) to serve as negative and positive controls, respectively, for the position of hepta-acyl lipid A. LptFG-depletion strain NR1113 was grown with (lane 3) or without (lanes 4 and 5) arabinose. [1-14C]Acetate was added either at the beginning of subculturing to label steady-state LPS (lanes 1–4) or after LptFG depletion to label de novo-synthesized LPS (lane 5). Equal amounts of radioactive material were spotted for each sample.