Table 2.

Proposed role of each mutation introduced into 8g8

8g8 mutations	Generation	Mutation group	Distance to S189 Oγ	Proposed role for 8g8 mutations
160V	1	2	17.0  Å	Stabilizes 64–74 and 414–420 loops. Allows 10 intramolecular H bonds.
L144M	1	1	17.8  Å	Stabilizes 265–275 loop. Interacts with M358V, H322Y, and L343.
M358V	1	1	11.9  Å	Shifts 265-275 and 315-324 loops (smaller shift than 56c8). Interacts with H322Y, L144M, and A343V.
Y370F	1	3	16.5  Å	Perhaps results in more favorable solvation.
A343V	2	1	25.0  Å	Alters packing among helices 9, 10, 11, and 15.
1437T	3	3	21.7  Å	Forms intramolecular H bond, stabilizing helix 17.
H322Y	4	1	20.8  Å	Positions 315–324 loop. Determines the interaction between 265-275 active site loop and 315-324 loop. Interacts with M358V, L144M, and A343V.
L313F	5	3	10.7  Å	Forms face-to-edge interaction with F314.
G412E	5	2	17.0  Å	Precedes 413–421 loop, which is unstructured in WT. Forms salt bridge with R415.
T73K	7	2	21.1  Å	In 65–75 loop, which is unstructured in WT. Forms salt bridge with E74.
T459S	7	4	28.4  Å	Thr and Ser both H bond to H456. Small cost of exposing a methyl group is saved.
A56V	8	4	25.8  Å	No observable changes.
A400T	8	3	7.8  Å	Thr-400 is in the active site.