Skip to main content
. Author manuscript; available in PMC: 2008 Apr 9.
Published in final edited form as: J Biol Chem. 2006 Jul 20;281(37):26774–26778. doi: 10.1074/jbc.C600171200

FIGURE 3. Kinetics of [Lys]Pg activation by wtSK and SKΔK414.

FIGURE 3

A, dependence of v1 on the total SK species concentration ([SK]o) obtained from reactions of 15 nM [Lys]Pg in the presence of 200 μM VLK-pNA and in the absence (●) and presence (○) of 10 mM 6-AHA and with SKΔK414 in the absence (■) and presence (□) of 10 mM 6-AHA. B, SK concentration dependencies of v2 rates for wtSK in the absence (●) and presence (○) of 10 mM 6-AHA and SKΔK414 in the absence (■) and presence (□) of 10 mM 6-AHA. Lines represent the fits by the equations described previously (5, 6) with the parameters given in the “Results and Discussion.” Activation reactions were performed and analyzed as described under “Experimental Procedures.”