TABLE 1. Relative metabolite abundance produced from the metabolism of capsaicin and nonivamide by human liver microsomes and recombinant human CYP2C9.

Incubations were performed as described under Materials and Methods. Relative metabolite abundance was determined as the percentage of total metabolite peak area obtained from selected ion monitoring LC/MS for M1 to M9 (n = 3) (Reilly et al., 2003a).

	HLM		CYP2C9
Metabolite Identification	Capsaicin	Nonivamide	Capsaicin	Nonivamide
	% of total metabolites		% of total metabolites
Macrocycle (M1)	22 ± 4	N.D.	34 ± 3	N.D.
ω-OH (M2)	18 ± 4	24 ± 4	1 ± 0.2	0.8 ± 0.1
ω-1-OH (M3)	8 ± 2	N.D.	< 0.1	N.D.
Diene or alkene (M4)	28 ± 5	4 ± 1	63 ± 5	5 ± 2
Others (M5–M9)	28 ± 14	71 ± 4	< 2	95 ± 4

HLM, human liver microsomes; N.D., not detected.