Figure 4.
Defects in adhesion and proliferation of cultured ILK-deficient keratinocytes. (A) Primary keratinocytes isolated from 3-d-old Ilkf/+;K14-Cre mice or Ilkf/f;K14-Cre littermates were plated at a density of 100,000 cells/well in 12-well culture dishes at t = 0. The number of cells/well and [3H]dThd incorporated into DNA (expressed as a percentage of [3H]dThd incorporated in Ilkf/+;K14-Cre keratinocytes at t = 0), were determined at the indicated intervals after initial seeding. The results represent the average + SEM of three experiments done in triplicate. * p < 0.05 (ANOVA). (B) Phase-contrast micrographs of live cultured keratinocytes from Ilkf/+;K14Cre mice or Ilkf/f;K14Cre littermates, 3 d after isolation and plating. Bar, 30 μm.