Table 1.

Summary of threshold cycle data for telomere amplification using Q-PCR

Ct	Telomere	GAP-43
UD	18.24 ± 0.18 (0.9865)	32.74 ± 0.5 (0.8533)
3 day	22 ± 0.22 (0.9795)***	33.08 ± 0.27 (0.8945)
6 day	18.49 ± 0.21 (0.9674)	33.02 ± 0.2 (0.9243)

The threshold cycle (Ct) was determined at the Y intercept for each of the samples. The relative amounts of telomeric DNA in each sample was calculated by DΔ (see Materials and Methods) and averaged, with R² values in parentheses. Levels at the third cell cycle were decreased by 14.7-fold and at differentiation by 0.66-fold, compared with undifferentiated cells. The difference between the Cts for partially differentiated telomeric DNA and the other samples was significant (***p < 0.001) by ANOVA analysis followed by Student's t test. (n = 3 independent experiments).