Figure 5.

Effect of a variety of filament-inducing conditions on induction of UME6. A wild-type strain was grown under non–filament-inducing conditions (YEPD medium at 30°C) and diluted into pre-warmed flasks containing either YEPD or the indicated filament-inducing media at 30°C (or 37°C where shown). Cells were harvested at the zero time point (immediately before induction) and at 30 min after induction for total RNA preparation. Northern analysis was carried out using 3 μg of RNA from each sample to assess transcript levels of the indicated genes. The ACT1 transcript, as well as ribosomal RNA (rRNA) are shown as loading controls.