. 1999 Oct 26;96(22):12316–12321. doi: 10.1073/pnas.96.22.12316

Table 1.

Kinetic parameters for aminoacylation of microhelix^Ala at pH 7.5, 25°C

Enzyme	k_cat, min⁻¹	K_M, μM	k_cat/K_M (relative)
465N	1.9	100	(1.0)
374N-Ad^ScQRS	0.09	7	0.68
374N-C^Arc1p	0.11	110	0.05

Kinetic parameters were determined over a microhelix^Ala range of 10−400 μM (465N), 5−400 μM (374N-C^Arc1p), or 2−20 μM (374N-Ad^ScQRS).