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. 2008 Apr;19(4):1485–1498. doi: 10.1091/mbc.E07-09-0878

Figure 2.

Figure 2.

Morphological analyses of stage 3 hippocampal neurons expressing EGFP, EGFP-P60-katanin, or EGFP-spastin. Control (A) and P60-katanin–overexpressing (B) neurons displayed similar morphologies, with 4–6 minor processes and one axon, whereas neurons overexpressing spastin (C and D) displayed more minor processes than control neurons. Quantitative analysis of the microtubule levels, the number of minor process, the length of minor processes and the length of axons are shown in E (n > 60). The microtubule levels in the P60-katanin–overexpressing and spastin-overexpressing neurons decreased 12% (p < 0.05) and 30% (p < 0.001) respectively compared with control neurons. There is a 70% increase of the number of minor processes in hippocampal neurons overexpressing spastin compared with control and P60-katanin–overexpressing neurons (p < 0.001). There is no significant difference in the length of minor processes and axons between any of the experimental groups (p > 0.5). Scale bar, 40 μm.