Figure 1.

Nop1p delocalizes from the nucleolus in rna14-1 and rna15-2 mutants. Wild-type (a) and mutant (b–d) cells expressing a Nop1p-GFP fusion protein were grown in glucose medium at 23°C, and then they were shifted to 37°C for 1 h. N, nucleoplasm; nu, nucleolus; arrows point to nuclear foci. Wild-type (e), rna14-1 (f) and rna15-2 (g) strains incubated at 37°C for 1 h were immunolabeled with anti-Nop1p antibody.