Figure 2.

Box C/D and box H/ACA snoRNP proteins, and U14 snoRNA, but not U3 snoRNA, redistribute from the nucleolus to nucleoplasmic foci in rna14-1 and rna15-2 mutants. The strains indicated were grown in glucose medium at 23°C (23°C panels) and then shifted to 37°C for 1 h (37°C panels). (A) Endogenous U14 and U3 snoRNAs were detected by in situ hybridization. (B) Endogenous Nop58p was detected by immunofluorescence. (C) Wild-type and mutant cells expressing a Gar1p-GFP fusion protein. Note that a fraction of Nop58p and Gar1p persists localized in the nucleolus (nu) at the nonpermissive temperature.