Figure 4.

Detection of polyadenylated RNA in combined Rna14p, Rna15p, and exosome mutant strains. Poly(A)⁺ RNA was detected by FISH using a Cy3-labeled oligo(dT)₅₀ probe. The indicated strains were grown at 23°C, and then they were incubated for 1 h at 37°C (a–m). The GAL::RRP41 (n and o), rna14-1/GAL::RRP41 (p), and rna15-2/GAL::RRP41 (q) strains were grown in YPD-2% galactose at 23°C, transferred to glucose medium at 23°C for 20 h, and then shifted to 37°C for 1 h. For comparison of fluorescence signals, all images were acquired with the same settings. d and e, k and l, and n and o show different exposures of the same cell.