Figure 6.

Rna14p and Rna15p are not necessary for 3′-end processing of U14. Total RNA was extracted from the indicated strains. Cells were grown at 23°C, and then they were incubated for 1 h at 37°C, as indicated. Strains with GAL-regulated alleles were grown in galactose medium at 23°C, transferred to glucose medium at 23°C for 20 h, and then shifted to 37°C for 1 h. (A) Northern hybridization with probes for U14 and U3 snoRNAs. The altered gel mobility in the rrp6Δ strain represents a failure in the 3′ trimming of the U14 snoRNA, as described previously (Allmang et al., 1999; van Hoof et al., 2000; Mitchell et al., 2003). (B) Total RNA recovered from cells incubated for 1 h at 37°C was deadenylated by treatment with RNase H in the presence (+lanes) or absence (−lanes) of oligo(dT). Note that there is almost no 3′-end extended and polyadenylated U14 in the rna14-1/rrp6Δ and rna15-2/rrp6Δ double mutants.