Figure 9.

FRAP analysis of Nop1-GFP in rna14-1 and rna15-2 single mutants and in rna14-1/rrp6Δ and rna15-2/rrp6Δ double mutants. Live cells were imaged at 23°C or 37°C, as indicated, using the 488-nm line of an Ar laser. Shown are images taken before, immediately after bleaching and after recovery for 30 s for rna15-2 at 23°C (A) and rna14-1/rrp6Δ at 37°C (B). The bleached region is outlined. The fluorescence intensity in the bleached region was measured and expressed as the normalized fluorescence (R). The recovery curves correspond to a pool of three different experiments, with at least 10 different cells analyzed per experiment. Error bars represent standard deviations. Fluorescence recovery curves are shown for the different strains at 23 and 37°C (C–F).