Null mutations in CLN1 and CLN2 restore polarized cortical recruitment of GFP-Ste5 and Bni1-GFP in fus3Δ cells. (A) Cortical recruitment of GFP-Ste5 in FUS3, fus3Δ, fus3Δ cln1Δ, and fus3Δ cln2Δ strains after α factor stimulation. FUS3 (EY1775), fus3Δ (EY1774), fus3Δ cln1Δ (EYL4684), and fus3Δ cln2Δ (EYL4649) strains harboring CUP1p-GFP-STE5 (pSKM21) were prepared and analyzed as in Figure 2B. Tally of GFP-Ste5 localization in different categories of cells and percentage of shmoos in total cells are shown below. (B) Recruitment of Bni1-GFP in FUS3, fus3Δ, fus3Δ cln1Δ, and fus3Δ cln2Δ strains after α factor stimulation. FUS3 (EY957), fus3Δ (EY1095), fus3Δ cln1Δ (EY1094), and fus3Δ cln2Δ (EY1093) strains harboring Bni1-GFP (EBL334) were prepared and analyzed as in Figure 4D. Tally of cortical recruitment of Bni1-GFP in different categories of cells and percentage of shmoos in total cells are shown below.