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. 2008 Apr;19(4):1739–1752. doi: 10.1091/mbc.E07-08-0757

Figure 6.

Figure 6.

Inactivation of Cdc28 stimulates cortical recruitment of Ste5. (A) The cdc28-4 mutant has an elongated morphology that is dependent on FUS3 KSS1. Comparison of cell morphology and GFP-Ste5 localization in cdc28-4 (EYL2190) and cdc28-4 fus3Δ kss1Δ (EYL2184) cells expressing CUP1p-GFP-STE5 pregrown at room temperature or 37°C for 3 h followed by 1-h induction in 500 μM CuSO4 to induce GFP-Ste5 expression, followed by further incubation at indicated temperature for 3 h without or with addition of 50 nM α factor. (B) Pattern of cortical recruitment of Ste5-3xGFP in cdc28-4, cdc28-4 fus3Δ, and cdc28-4 bni1Δ cells. Strains cdc28-4 (EYL4705), cdc28-4 fus3Δ (EYL4710), and cdc28-4 bni1Δ (EYL4711) with integrated Ste5-3xGFP were pregrown at room temperature overnight, and then they were shifted to prewarmed 37°C medium for 3 h followed by incubation at same temperature. (C) Pattern of different Ste5-3xGFP pools in cdc28-4, cdc28-4 fus3Δ, and cdc28-4 bni1Δ cells before and after α factor stimulation. Strains cdc28-4 (EYL4705), cdc28-4 fus3Δ (EYL4710), and cdc28-4 bni1Δ (EYL4711) were grown at room temperature. The different arrowheads point to speckles, blobs, and shmoo tip pools of Ste5-3xGFP.