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. 2008 Apr;19(4):1282–1294. doi: 10.1091/mbc.E07-07-0659

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© 2008 by The American Society for Cell Biology

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Figure 7. — VPS68 is required for two transport pathways out of the late endosome. Wild-type (SNY128) and vps68 (BWY12) strains containing the vps27Δ mutation and carrying pHY5 for the GAL1-driven expression of VPS27 were grown to log phase in raffinose medium. Aliquots removed 0, 45, 60, 75, and 90 min after the addition of galactose were processed for the localization of integrated, HA-tagged Vps10p and endogenous Vph1p by double-label immunofluorescence microscopy. (A) Representative images of cells fixed 0, 60, and 90 min after galactose addition. (B and C) Localization of Vph1p (B) and Vps10p (C) was scored for 100–200 cells at each time point. Dark gray bars, vacuole; black bars, class E compartment; light gray bars, dispersed/ambiguous. Bar, 2 μm.