Table 1.
Yeast strains used in this study
| Straina | Relevant genotype | Derivation/source |
|---|---|---|
| BY4743 | MATa/α LYS2/lys2Δ0 ura3Δ0/ura3Δ0 his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 | Brachmann et al. (1998) |
| YEF473 | MATa/α lys2–810/lys2–810 ura3–52/ura3–52 his3Δ-200/his3Δ-200 trp1Δ-63/trp1Δ-63 leu2Δ-1/leu2Δ-1 | Longtine et al. (1998) |
| KKT33 | MATalys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 DNF1-GFP::HIS3MX6 | This study |
| KKT39 | MATalys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 DNF1-3HA::HIS3MX6 | This study |
| KKT58 | MATα lys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 KEX2-GFP::HIS3MX6 | This study |
| KKT61 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 | This study |
| KKT62 | MATalys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 SEC7-mRFP1::HIS3MX6 | This study |
| KKT70 | MATα LYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 | This study |
| KKT72 | MATα LYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 fpk1Δ::HphMX4 | This study |
| KKT75 | MATα lys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 MET15 MYO2-GFP::HIS3MX6 | This study |
| KKT102 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 lem3Δ::KanMX6 | This study |
| KKT117 | MATα lys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 MET15 HphMX4::PGAL1-3HA-CDC50 fpk1Δ::KanMX6 | This study |
| KKT127 | MATα LYS2 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 HphMX4::PGAL1-3HA-CDC50 | Sakane et al. (2006) |
| KKT266 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 fpk2Δ::KanMX6 | This study |
| KKT268 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| KKT287 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 HphMX4::PGAL1-3HA-CDC50 | This study |
| KKT293 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 HphMX4::PGAL1-3HA-CDC50 lem3Δ::KanMX6 | This study |
| KKT330 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 HIS3MX6::PGAL1-3HA-CDC50 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| KKT331 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 HIS3MX6::PGAL1-3HA-CDC50 fpk2Δ::KanMX6 | This study |
| KKT332 | MATalys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 DNF1-GFP::HIS3MX6 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| KKT334 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 DNF2-GFP::HIS3MX6 | This study |
| KKT336 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 DNF2-GFP::HIS3MX6 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| KKT340 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 DNF1–3HA::HIS3MX6 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| KKT342 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 DNF2–3HA::HIS3MX6 | This study |
| KKT344 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 DNF2–3HA::HIS3MX6 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| KKT345 | MATα LYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 HIS3MX6::PGAL1-GST-FPK1ΔN | This study |
| KKT346 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 KEX2-GFP::HIS3MX6 lem3Δ::KanMX6 | This study |
| KKT347 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 KEX2-GFP::HIS3MX6 HphMX4::PGAL1-3HA-CDC50 | This study |
| KKT348 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 KEX2-GFP::HIS3MX6 HphMX4::PGAL1-3HA-CDC50 lem3Δ::KanMX6 | This study |
| KKT349 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 KEX2-GFP::HIS3MX6 HIS3MX6::PGAL1-3HA-CDC50 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| KKT350 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 MET15 KEX2-GFP::HIS3MX6 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| KKT351 | MATaLYS2 ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 MYO2-GFP::HIS3MX6 lem3Δ::KanMX6 | This study |
| KKT353 | MATalys2Δ0 ura3Δ0 his3Δ1 leu2Δ0 MET15 MYO2-GFP::HIS3MX6 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| YKT1363 | MATα lys2-810 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DNF1-13myc::TRP1 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| YKT1364 | MATα lys2-810 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DNF2-13myc::TRP1 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| YKT1365 | MATα lys2-810 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DNF3-13myc::TRP1 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| YKT1366 | MATα lys2-810 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 DRS2-13myc::TRP1 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
| YKT1367 | MATα lys2-810 ura3-52 his3Δ-200 trp1Δ-63 leu2Δ-1 NEO1-13myc::TRP1 fpk1Δ::HphMX4 fpk2Δ::KanMX6 | This study |
a KKT strains are isogenic derivatives of BY4743. YKT strains are isogenic derivatives of YEF473.