Figure 7.

Rate of actomyosin ring constriction slows down in pxl1Δ mutant cells. (A) Percentage of cells with Rlc1p ring is higher in pxl1Δ mutant. Wild-type and pxl1Δ mutant cells expressing rlc1-GFP were grown at 24°C and observed by fluorescence microscopy. The number of cells with Rlc1p ring was quantified (n = 500). (B) Rate of ring constriction is constant in both wild-type and pxl1Δ mutant cells. Wild-type cells expressing rlc1-GFP were grown at 24°C and observed by time-lapse confocal microscopy. Z-stack images were taken at different time points. 3D-reconstruction images are shown at a 45° angle. The diameter of the Rlc1p ring was measured and plotted over time. Two examples from wild-type and pxl1Δ mutant cells are shown, and the rate of ring constriction was calculated using the stated formula. d_n represents the diameter of the actomyosin ring at the time t_n. (C) Rate of ring constriction is slower in pxl1Δ mutant cells. Wild-type and pxl1Δ mutant cells expressing rlc1-GFP were treated and observed as in B, and the calculation of the rate of ring constriction was based on the formula in B (n = 10).