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. 2008 Apr;19(4):1680–1692. doi: 10.1091/mbc.E07-07-0715

Figure 8.

Figure 8.

LIM domains are required for Pxl1p function. (A) pxl1-limΔ cells show a cell separation phenotype as in pxl1Δ. Wild-type, pxl1Δ, and pxl1-limΔ cells were grown at 24°C and observed by light microscopy. (B) LIM domains can rescue the pxl1Δ phenotype. pxl1Δ cells bearing the plasmids pREP81, pREP81-pxl1, pREP81-pxl1-N, and pREP81-pxl1-C were grown at 24°C. Samples were taken and stained with aniline blue to visualize septa. (C) Quantification of the number of septa. Cells from B were quantified for the number of septa (n = 500). (D) Localization of N- and C-terminal parts of Pxl1p. pxl1Δ cells bearing the plasmids pREP81-GFP-pxl1, pREP81-GFP-pxl1-N, and pREP81-GFP-pxl1-C were grown at 24°C and observed by fluorescence microscopy. Images with Calcoflour white staining of the cell wall are shown at the bottom. Arrow indicates the nucleus localization of GFP-Pxl1p-C. Scale bar, 5 μm.