Figure 2.

Transcriptional regulation of apy-1 under stress conditions. (A) Northern blot analysis of total RNA from untreated and 5 μg/ml tunicamycin (Tun)- or 6% ethanol (EtOH)-, or 25°C (T)-treated N2 animals for 6 h. (B) Northern blot analysis of total RNA from untreated and 5 μg/ml tunicamycin (Tun)-treated animals of the indicated mutant strains, each lacking a different UPR activation pathway. Quantification of the radiolabeled signal on the blot is shown in bottom part of each panel. The hybridization signal for each strain in the untreated condition was set as 1. These data represent one of three independent experiments giving the same result; error bars, SD.