Figure 3.

Induction of an ER stress reporter gene by apy-1 RNAi treatment. (A) Fluorescence micrographs of SJ4005 worms, containing an integrated copy of hsp-4::gfp, a green fluorescent protein transcriptional reporter driven by the hsp-4 promoter, untreated (UT), tunicamycin-treated (Tun; 5 μg/ml), or apy-1(RNAi) by feeding animals. Fluorescent images were obtained using the same exposure for all treatments. (B) Northern blot analysis of total RNA from N2 and apy-1(RNAi) worms. The blot was hybridized with hsp-4 probe that detects the endogenous gene. Quantification of the radiolabeled signal is shown in the bottom part of the panel. These data represent one of three independent experiments giving similar results; error bars, SD. (C) Fluorescence micrographs of SJ30 worms, containing in a ire-1 background an integrated copy of hsp-4::gfp, fed with bacteria carrying the empty vector (control) or fed with bacteria expressing apy-1(RNAi). (D) Fluorescence micrographs of SJ4005 worms fed with bacteria carrying the empty vector (control) or fed with bacteria expressing uda-1(RNAi). Fluorescent images were obtained using the same exposure for all treatments.