Figure 7.

RASSF7 localizes to the centrosomes. (A) Embryos were injected with GFP-RASSF7 at the two-cell stage, cultured until stage 10, fixed, sectioned, and stained as described in materials and methods. GFP-RASSF7 (green) colocalized with γ-tubulin (red) during interphase and prophase (arrow head and arrow, respectively) and during metaphase and anaphase (arrows). Sections were counterstained with DAPI (blue). The localization has been repeated in more than three independent experiments. (B) RASSF7-HA (red) and GFP-RASSF7 (green) exhibit the same localization in stage 10 embryos. Interphase cells shown. (C) Later stage embryos (stage 20) also have centrosome localized GFP-RASSF7 (green), as indicated by γ-tubulin (red). Image shows the neural tube of a stage 20 embryo. VC, ventricular cavity. All bars, 10 μm. RASSF7 colocalizes with γ-tubulin throughout the cell cycle.