Figure 6.

Misalignment defects in Bub3/BubR1-, Bub1/BubR1-, and Bub1/Bub3-RNAi cells. (A) Immunofluorescence images of control and siRNA-treated prometaphase cells stained with Bub3, Bub1, or BubR1 antisera as indicated and (B) immunoblots of lysates from siRNA-transfected cells, with α-tubulin acting as loading control, showing that double repressions were efficient. Scale bar, 5 μm. (C) Chromosome misalignment in cells transfected with siRNA as indicated and treated with MG132. Chromosomes were counted as misaligned if they were located outside the 40% central spindle area of the mitotic spindle. Error bars, SEM of three independent experiments (>250 kinetochores, >5 cells).