Effects of BD3 on part of the proteins related to Fc receptor signal pathway of CHO-IIA cells. CHO-IIA cells transfected with GFP-BD3 (a, d, and g) or cotransfected with GFP-BD3 and RFP-coronin (i) were allowed to undergo for phagocytosis for 10 min before fixation. Endogenous SEPT2 was immunostained by SEPT2 antibody (b, e, and h), actin by Rhodamine-phalloidin (c) and phosphotyrosine by mouse anti-phosphotyrosine mAb (f). Open arrows, polystyrene beads at unsealed phagocytic cups; Bars, 5 μm.