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. 2008 Mar 18;8:86. doi: 10.1186/1471-2148-8-86

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Copyright ©2008 Göker and Grimm; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neighbor-Net splits graph based on PBC-inferred distances: Acer section Acer. This graph largely agrees with the results of [9]: Individuals (bold labels) of the same taxon ('species') and intrasectional group (A0 to B4) cluster together. The position of potentially hybrid individuals (hd 1, of 2, sv4b) is pronounced and is directly visible from the graph because of the large proximal box-like portions. These hybrid individuals exhibit ITS clones of different evolutionary origin (ITS homoeologues), highlighted for the example of individual sv4b. Clones of this individual either represented A. ibericum-type or group B1-type homoeologues. Related edge bundles (reddish, bluish) can be addressed in the graph. Green squares correspond to individuals that exhibited recombinant (chimeric) clones (see text).