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Effect of PSK on PBL proliferation. PBLs were cultured with PSK or IL-2 or with PSK+IL-2. PBLs (50 × 104 cells) were seeded in quadruplicate in 96-well plates. The proliferation was determined by BrdU incorporation and absorbance measurement. PSK showed a synergistic effect with IL-2, increasing PBL proliferation. PSK alone did not induce PBLs proliferation. Each column represents the mean of five independent experiments ± SD. *P < 0.001 versus control.
