TABLE 1.

Detailed Gradient Settings for the Strong Cation Exchange and the Reverse Phase Chromatography: Valve Switching Points for the Autosampler Micro 6-Port Valve and the Micro 10-Port Valve

NANOFLOW PUMP
Time [min]	0	10	70	70.01	85	145	145.01	160	220	220.01	235	295	295.01	310	370	370.01	385
% Solvent B	5	5	65	5	5	65	5	5	65	5	5	65	5	5	65	5	5
Time [min]	445	445.01	460	520	520.01	535	595	595.01	610	675	675.01	685	745	745	760	820	820.01
% Solvent B	65	5	5	65	5	5	65	5	5	65	5	5	65	5	5	65	5
CAPILLARY PUMP
Time [min]	0	15	15.01	30	30.01	90	90.01	105	105.01	165	165.01	180	180.01	240	240.01	255	255.01	315	315.01	330	330.01
% Solvent B	0	0	0	2.5	0	0	2.5	5	0	0	5	7.5	0	0	7.5	10	0	0	10	15	0
Time [min]	390	390.01	405	405.01	465	465.01	480	480.01	540	540.0	555	555.01	615	615.01	630	630.01	690	690.01	705	705.01	820.01
% Solvent B	0	15	20	0	0	20	30	0	0	30	50	0	0	50	100	0	0	100	100	0	0
10-PORT VALVE
Switch Position	1	2	1	2	1	2	1	2	1	2	1	2
Time [min]	0	10	85	160	235	310	385	460	535	610	685	760
MICRO WELL-PLATE AUTOSAMPLER 6-PORT VALVE
Mainpass time [min]	0	90	165	240	315	390	465	540	615	695	765
Bypass time [min]	30	105	180	255	330	405	480	555	630	705