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ESI-MS spectrum of apo-siderocalin. The mass spectrum was obtained by averaging 50 scans acquired during elution of free siderocalin peak shown in Figure 1. The inset displays the deconvoluted spectrum that resulted upon MaxEnt1 processing of the ESI-MS spectrum. The calculated relative molecular weight of the protein matched the expected molecular weight of 20,675 Da, calculated according to the sequence of the protein.
