FIGURE 4.
Chinese hamster ovary phosphorylation of NSATCHSEDS(PO4) DLEIDALEGPR. Collison-induced dissociation spectrum, in information-dependant acquisition mode of the parent ion at m/z 1198.5 is shown, representing amino acids 287–301 plus one phosphate. The y13 through y16 ions confirm the presence of a dehydroalanine resulting from loss of phosphate upon fragmentation at position 296.