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Doubly charged ion showing phosphorylation of phosducin-like protein at one of three consecutive serines, Ser18–Ser20. A: Collison-induced dissociation (CID) of the +2 charged parent ion at m/z 1281 chosen automatically in information-dependant acquisition mode. This species represents amino acids 13–32 with one phosphate. The y8 ion excludes the possibility of phosphorylation at Ser25. B: CID of the same parent ion chosen by hand for LC-MS/MS.
